The Cowballs are spheres that have cow patterns and make cow sounds. They first appear in Long, Short, Roll in the short, Bob on a Roll where Bob the Ball, Plush, and Mr. String play cowboy and heard them up. They and their debut short would also appear in Bob's own episode and Roll, Spin, Pop. However, they also made an appearance in Hit the Hay from Hit, Miss, Bump where Bob the Ball and Little Cup are seen playing cowboy again.
Not much is known about them other than they act simaler to real cows, and they run from others if being chased. It is also unknown what they eat, if there all female like real cows, or if there are some male cows.